CAPITAL PURCHASE OF Next Generation Sequencer (NGS)

HSE are looking to procure a Next Generation Sequencing (NGS) system for routine bacterial and fungal identification purposes that will require the partial sequencing of targeted genes, rather than the whole genome work. The system must be capable of identifying microorganisms to species level either from purified colonies (culture) or from mixed populations of microorganisms without the need for culture; the latter based on direct DNA extraction and target gene amplification from mainly environmental samples (e.g. soil, water, air). Based on current and expected future identification requirements, HSE expect that most if not all bacterial work will focus on the 16S rRNA gene (16S rDNA seq…

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