CMOS Camera and Software for CryoTEM

Cutting edge electron cryo-microscopy requires CMOS-based detectors and state-of-the-art imaging software to enable automated collection of high-quality data at high throughput rates. The camera and software are intended for a 120 kV FEI Tecnai Spirit transmission electron microscope (TEM) with cryo-capability. We will record images and tomographic tilt series of single particle protein samples, as well as more complex heterogeneous samples at both room temperature and under cryo conditions. Due to the side-entry cryo holder fitted to our microscope, a fast read-out camera is required in order to account for specimen/ stage drift. The camera must therefore be bottom-mounted and capable of hi…

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