MIROCALS Study Flow cytometry cell subset numeration in human whole blood and monitoring of pNF-H in CSF and plasma samples by ELISA

The Service Provider is requested to monitor the absolute number and frequency of circulating white blood cell subsets including FOXP3+ regulatory T cells (Tregs), CD3+, CD4+, CD8+, B, and NK cells in EDTA whole blood samples and the circulating pNF-H biomarker in CSF and plasma. For whole blood samples, cell subsets should be analysed by flow cytometry. There will be 216 patients and 5 time points per patient (1080 samples in total). For plasma and CSF samples, the pNF-H should be monitored using a commercial ELISA kit (Biovendor). There will be 3 plasma samples + 3 CSF samples per patient from 216 patients (2 x 3 x 216 = 1296 samples in total. All procedures will need to be validated,…

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